elisa timp2 kit  (Cusabio)

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: Identification of Key Genes Involved in FDM Through Transcriptome Sequencing Analysis . Note: (A) The volcano plot illustrating DEGs in the transcriptome sequencing dataset, where red points represent log2FC > 2 and P value < 0.05, blue points represent log2FC < −2 and P value < 0.05, and gray points indicate genes with no significant differences (n = 3 for Control group, n = 3 for FDM group); (B) Venn diagram showing the intersection between DEGs and FDM-related genes from the genecards database; (C) Heat map displaying the DEGs in the intersection; (D) PPI network of candidate genes encoded by String database; (E) Degree values of protein interactions encoded by candidate genes; (F) Comparative graph of Timp2 cell expression across samples in each group (n = 3 for Control group, n = 3 for FDM group). ∗∗∗ indicates P < 0.001 in pairwise comparisons between the two groups. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Sequencing, Control, Expressing

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: Impact of Timp2 mRNA Transfection on SSCs . Note: (A) Timp2 mRNA expression levels were assessed by RT-PCR at 1, 4, and 7 days post-transfection; (B) Timp2 protein expression levels were analyzed by Western Blot at 1, 4, and 7 days post-transfection; (C) Flow cytometry analysis of SSCs' cell surface marker expression 24 h post-transfection; (D) Effects of Timp2 mRNA transfection on SSCs' adipogenic, chondrogenic, and neurogenic differentiation were evaluated using Oil Red O staining, Alcian Blue staining, and immunofluorescence staining (scale bar = 50 μm); (E) Cell proliferation capacity of SSCs post-Timp2 mRNA transfection was examined using CCK-8 assay. "ns" indicates no significant difference between the two groups, ∗∗∗ P < 0.001. Cell experiments were repeated three times. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry, Marker, Staining, Immunofluorescence, CCK-8 Assay

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: Viability, Proliferation, and Apoptosis of SSCs Timp2 in Gtn-HPA Hydrogels . Note: (A) LIVE/DEAD staining was used to assess the viability of SSCs Timp2 in Gtn-HPA hydrogels, with white arrows indicating dead cells; (B) Immunofluorescence was employed to examine the proliferation of SSCs Timp2 in Gtn-HPA hydrogels; (C) Immunofluorescence was utilized to evaluate the apoptosis of SSCs Timp2 in Gtn-HPA hydrogels. "ns" indicates no significant difference between the two groups, and the cell experiments were repeated three times.

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Staining, Immunofluorescence

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: Effects of SSCs Timp2 -GH on Proliferation, Apoptosis, and Differentiation of Sclera Fibroblasts . Note: (A) Cell proliferation in each group was assessed by flow cytometry; (B) Cell apoptosis in each group was evaluated using flow cytometry; (C) Proportion of α-SMA-positive cells in each group was determined by flow cytometry; (D) Expression of α-SMA and COL1A1 in cells of each group was quantified by ELISA. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. All cellular experiments were replicated three times.

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: PK/PD Study of SSCsTimp2 . Note: (A) After subcutaneous injection of radioactive-labeled RNA in SSCsNC and SSCsTimps suspensions, tissue uptake in organs was assessed using radioactive analysis (percentage of injected dose per gram tissue (%ID/g)) to evaluate the pharmacokinetics of SSCsNC and SSCsTimps; (B) Pharmacodynamics study of Timp2 expression in the sclera at 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 h after SSCsNC suspension and SSCsTimps suspension administration. N = 6/group.

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Injection, Labeling, Drug discovery, Expressing, Suspension

Journal: Materials Today Bio

Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice

doi: 10.1016/j.mtbio.2025.101942

Figure Lengend Snippet: Effects of SSCs Timp2 -GH on improving myopia management in the sclera . Note: (A–C) Interocular differences in refraction (A), AL (B), and VCD (C) among the groups of mice (right eye minus left eye); (D) Histological examination of scleral tissues with H&E staining (red arrowheads indicate the sclera) and quantitative analysis of scleral thickness (scale bar = 50 μm); (E) Representative images of transverse (top) and longitudinal (bottom) sections of the posterior sclera in mice from each group, TEM images, and quantitative analysis of collagen fiber diameter (scale bar = 500 nm). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; each group consisted of 6 mice. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: TIMP2 protein expression in the sclera was measured using TIMP2 ELISA Kits (EMTIMP2, Thermo Fisher, USA) following the manufacturer's instructions.

Techniques: Staining

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: Inflammatory cytokine/chemokine profiles induced by C. acnes subtypes.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Control

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: Comparative analysis of IL8 and TIMP2 expression in normal skin, acne-affected, inflamed, and pigmented sites. IL8 and TIMP2 protein expression were quantified from (1) non-acneic skin, (2) normal skin, (3) sites of inflammation, and (4) sites of pigmentation in acneic skin by ELISA. * p < 0.05 versus control group; Student’s t -test. Data are expressed as the mean ± SEM.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: IL8 and TIMP2 regulatory effects of chlorogenic acid and taurine in cultured HaCaT cells. The synergistic effect of chlorogenic acid and taurine on ( A ) IL8 inhibition, and ( B ) TIMP2 activation after 24 h of co-treatment with heat-killed C. acnes . * p < 0.05, ** p < 0.01, *** p < 0.001 relative to the control group; Student’s t -test. Data are expressed as mean ± SEM.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Cell Culture, Inhibition, Activation Assay, Control